After the instrument is preheated, the wavelength hand wheel is rotated and the desired wavelength is aligned with the calibration line.

Usage: (1) instrument preheating. Open the cover of the sample room (automatic closing of light door). Turn on the power supply, the indicator light is on, and the instrument is preheated for 20 min. The selection switch is placed in the "T" knob, so that the number is displayed as "0". (2) rotate the wavelength handwheel and mark the desired wavelength at the scale line. (3) placing the colorimetric dish containing the solution in the coloring rack, so that the reference solution is placed in the optical path. (4) cover the cover of the sample room and adjust the transmittance "100%T" knob, so that the number is displayed as "100.0T". (if you do not display 100%T, you can press it one time. (5) Measurement of absorbance A: After the instrument is set to 0 and 100%, the switch of choice is switched to A zero knob, and the digital display should be ".000". Then the pull rod is pulled out so that the measured solution is placed into the optical path, and the digital display value is the absorbance A of the sample. (6) after the measurement is completed, the sample chamber cover is opened first, then the power supply is cut off. The coloured cups should be cleaned and stored.

There are two main types of atomizer, that is, flame atomizer and electrothermal atomizer. There are many kinds of flames, and the air acetylene flame is widely used nowadays. Graphite furnace atomizer is widely used in electrothermal atomizer, so atomic absorption spectrophotometer, flame atomic absorption spectrophotometer and atomic absorption spectrophotometer with graphite furnace. The atomization temperature of the former is between 2100 and 2400 degrees, and the latter is between 2900 and 3000 degrees.

Flame atomic absorption spectrometer, the use of air-acetylene determination of more than 30 elements, if the use of nitrous oxide-acetylene flame, the determination of more than 70 elements. However, the safety of Nitrous Oxide acetylene flame is poor and its application is not widespread. Air-acetylene flame atomic absorption spectrometry can generally detect PPm (10-6), the precision of about 1%.

Operating procedures for atomic absorption spectrophotometer:

Turn on the computer - turn on the mainframe power - double-click on the icon for self-test - wait for the completion of self-test element lamp selection - according to the wizard prompt for peak search operation.

Flame method: Click on the icon to set the sample - click on the icon to set the parameters - click on the icon to adjust the position of the atomizer - turn on the air compressor power supply (pressure 0.25-0.3 MPa) - turn on the acetylene cylinder switch (pressure adjustment to about 0.06MPa) - click on the icon - click on the icon to balance energy automatically. Click click icon to start the measurement. Turn off acetylene, air compressor and turn off main engine after measurement.

Graphite furnace method: click on the icon to set the sample - click on the icon to set the parameters - click on the icon to adjust the position of the atomizer - Open argon (pressure 0.6-0.8 MPa) - cooling water - graphite furnace power - click on the furnace body to open, install the graphite tube, click on the furnace body after installation - click on the icon to adjust energy - click on the settings Set up temperature program. Click on empty burn three times, click to start measuring. After measuring, turn off argon, cooling water, graphite furnace power and turn off the main engine.

Manufacturers of atomic absorption spectrophotometer